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原文摘要

The rapid growth of scientific literature and the high heterogeneity of biological terminology and mechanistic descriptions make it difficult for researchers to integrate scattered research evidence, identify connections among biological events, and construct mechanistic networks. Here, we developed a high-throughput human-AI hybrid framework, AOP-Smart-Finder, for biological event discovery, relationship mining, and Adverse Outcome Pathway (AOP) construction and evidence mapping from large-scale literature. This framework integrates Large Language Models (LLMs), AOP-Smart retrieval-augmented generation (RAG) technology, and Python-based automation, supporting two analysis modes: one based on a single compound and the other based on a predefined set of biological events. In the di(2-ethylhexyl) phthalate (DEHP) case, the system processed 12,983 articles within 30 min and included 1,093 of them, from which it extracted 1,589 biological events (including 458 official key events [KEs] and 1,131 candidate KEs) and 2,519 key event relationships (KERs). In the amyotrophic lateral sclerosis (ALS) case, the system identified 55 stressors from 437 articles and extracted 278 biological events and 548 event relationships. Following manual curation and Weight of Evidence (WoE) evaluation, a mechanistic network comprising 16 KEs and 23 KERs was ultimately constructed. This framework can either use existing AOPs as knowledge guidance for mechanistic expansion and evidence supplementation, or discover novel event connections and construct mechanistic networks around user-specified research topics. It provides a high-throughput, traceable, human-AI hybrid methodology for complex biological mechanism elucidation and AOP discovery driven by large-scale literature.